Mio pmn User Manual download pdf (Page 20)

20

+1 n NaOH ad pH 7,4

HBS (HEPES-Buffered Saline)-Puffer (0,5l):

500 ml Aqua Dest.

+2,383 g HEPES

+4 g NaCl

+1 n NaOH ad pH 7,6

HEPES (N-2-Hydroxyethylpiperazine-N!-2-ethanesulfonic acid); MW 238,3; pKa 7,5 bei

25°C, Sigma (Deisenhofen, Deutschland)

PBS (Phosphate-Buffered Saline)-Puffer ohne Calcium und Magnesium(1l):

8,0 g NaCl

+0,2 g KCl

+0,2 g KH

2

PO

4

+1,5 g Na

2

HPO

4

x2 H

2

O

ad 1000 ml Aqua Dest.

1n NaOH ad pH 7,4

PBS (Phosphate-Buffered Saline)-Puffer mit Calcium und Magnesium(1l):

8,0 g NaCl

+0,2 g Kcl

+0,2 g KH

2

PO

4

+1,5 g Na

2

HPO

4

x2 H

2

O

+0,1 g MgCl

2

x6H

2

O

ad 1000 ml Aqua Dest.

1 n NaOH ad pH 7,4

+10 ml CaCl

2

-Lösung (1,5g CaCl

2

ad 100 ml Aqua Dest.)

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